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vertical diffusion chamber (vdc  (Harvard Bioscience)

 
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    Harvard Bioscience vertical diffusion chamber (vdc
    Δ rsbW demonstrates increased cell adhesion but decreased virulence. (A) WT C. difficile , Δ rsbW , and Δ rsbW + rsbW adherence to epithelial cells across 3, 6, 12, and 24 h after infection in an in vitro <t>vertical</t> <t>diffusion</t> chamber <t>(VDC)</t> infection model ( N = 3, with 3 technical replicates/experiment). (B) Survival curve of Galleria mellonella infected with C. difficile WT, Δ rsbW , and Δ rsbW + rsbW strains across 24 and 72 h (N = 5, with 8 larvae/strain/time point). (C) Adherent bacterial population recovered from infected G. mellonella gut enumerated with CFU/mL on brain-heart infusion with 0.5% (wt/vol) yeast extract and 0.1% (wt/vol) l -cysteine (BHI-S) agar. Error bars indicate SD. n.s., no significance; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, as determined by Mann-Whitney U test and log rank Mantel Cox test for the survival curve.
    Vertical Diffusion Chamber (Vdc, supplied by Harvard Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vertical+diffusion+chamber+%28vdc/pmc10210984-277-19-23?v=Harvard+Bioscience
    Average 90 stars, based on 1 article reviews
    vertical diffusion chamber (vdc - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Regulatory Role of Anti-Sigma Factor RsbW in Clostridioides difficile Stress Response, Persistence, and Infection"

    Article Title: Regulatory Role of Anti-Sigma Factor RsbW in Clostridioides difficile Stress Response, Persistence, and Infection

    Journal: Journal of Bacteriology

    doi: 10.1128/jb.00466-22

    Δ rsbW demonstrates increased cell adhesion but decreased virulence. (A) WT C. difficile , Δ rsbW , and Δ rsbW + rsbW adherence to epithelial cells across 3, 6, 12, and 24 h after infection in an in vitro vertical diffusion chamber (VDC) infection model ( N = 3, with 3 technical replicates/experiment). (B) Survival curve of Galleria mellonella infected with C. difficile WT, Δ rsbW , and Δ rsbW + rsbW strains across 24 and 72 h (N = 5, with 8 larvae/strain/time point). (C) Adherent bacterial population recovered from infected G. mellonella gut enumerated with CFU/mL on brain-heart infusion with 0.5% (wt/vol) yeast extract and 0.1% (wt/vol) l -cysteine (BHI-S) agar. Error bars indicate SD. n.s., no significance; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, as determined by Mann-Whitney U test and log rank Mantel Cox test for the survival curve.
    Figure Legend Snippet: Δ rsbW demonstrates increased cell adhesion but decreased virulence. (A) WT C. difficile , Δ rsbW , and Δ rsbW + rsbW adherence to epithelial cells across 3, 6, 12, and 24 h after infection in an in vitro vertical diffusion chamber (VDC) infection model ( N = 3, with 3 technical replicates/experiment). (B) Survival curve of Galleria mellonella infected with C. difficile WT, Δ rsbW , and Δ rsbW + rsbW strains across 24 and 72 h (N = 5, with 8 larvae/strain/time point). (C) Adherent bacterial population recovered from infected G. mellonella gut enumerated with CFU/mL on brain-heart infusion with 0.5% (wt/vol) yeast extract and 0.1% (wt/vol) l -cysteine (BHI-S) agar. Error bars indicate SD. n.s., no significance; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, as determined by Mann-Whitney U test and log rank Mantel Cox test for the survival curve.

    Techniques Used: Infection, In Vitro, Diffusion-based Assay, MANN-WHITNEY



    Similar Products

    vertical diffusion chamber (vdc  (Harvard Bioscience)
    90
    Harvard Bioscience vertical diffusion chamber (vdc
    Δ rsbW demonstrates increased cell adhesion but decreased virulence. (A) WT C. difficile , Δ rsbW , and Δ rsbW + rsbW adherence to epithelial cells across 3, 6, 12, and 24 h after infection in an in vitro <t>vertical</t> <t>diffusion</t> chamber <t>(VDC)</t> infection model ( N = 3, with 3 technical replicates/experiment). (B) Survival curve of Galleria mellonella infected with C. difficile WT, Δ rsbW , and Δ rsbW + rsbW strains across 24 and 72 h (N = 5, with 8 larvae/strain/time point). (C) Adherent bacterial population recovered from infected G. mellonella gut enumerated with CFU/mL on brain-heart infusion with 0.5% (wt/vol) yeast extract and 0.1% (wt/vol) l -cysteine (BHI-S) agar. Error bars indicate SD. n.s., no significance; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, as determined by Mann-Whitney U test and log rank Mantel Cox test for the survival curve.
    Vertical Diffusion Chamber (Vdc, supplied by Harvard Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vertical+diffusion+chamber+%28vdc/pmc10210984-277-19-23?v=Harvard+Bioscience
    Average 90 stars, based on 1 article reviews
    vertical diffusion chamber (vdc - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Δ rsbW demonstrates increased cell adhesion but decreased virulence. (A) WT C. difficile , Δ rsbW , and Δ rsbW + rsbW adherence to epithelial cells across 3, 6, 12, and 24 h after infection in an in vitro vertical diffusion chamber (VDC) infection model ( N = 3, with 3 technical replicates/experiment). (B) Survival curve of Galleria mellonella infected with C. difficile WT, Δ rsbW , and Δ rsbW + rsbW strains across 24 and 72 h (N = 5, with 8 larvae/strain/time point). (C) Adherent bacterial population recovered from infected G. mellonella gut enumerated with CFU/mL on brain-heart infusion with 0.5% (wt/vol) yeast extract and 0.1% (wt/vol) l -cysteine (BHI-S) agar. Error bars indicate SD. n.s., no significance; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, as determined by Mann-Whitney U test and log rank Mantel Cox test for the survival curve.

    Journal: Journal of Bacteriology

    Article Title: Regulatory Role of Anti-Sigma Factor RsbW in Clostridioides difficile Stress Response, Persistence, and Infection

    doi: 10.1128/jb.00466-22

    Figure Lengend Snippet: Δ rsbW demonstrates increased cell adhesion but decreased virulence. (A) WT C. difficile , Δ rsbW , and Δ rsbW + rsbW adherence to epithelial cells across 3, 6, 12, and 24 h after infection in an in vitro vertical diffusion chamber (VDC) infection model ( N = 3, with 3 technical replicates/experiment). (B) Survival curve of Galleria mellonella infected with C. difficile WT, Δ rsbW , and Δ rsbW + rsbW strains across 24 and 72 h (N = 5, with 8 larvae/strain/time point). (C) Adherent bacterial population recovered from infected G. mellonella gut enumerated with CFU/mL on brain-heart infusion with 0.5% (wt/vol) yeast extract and 0.1% (wt/vol) l -cysteine (BHI-S) agar. Error bars indicate SD. n.s., no significance; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, as determined by Mann-Whitney U test and log rank Mantel Cox test for the survival curve.

    Article Snippet: The Snapwell inserts, containing polarized Caco-2, HT29, and CCD 18co myofibroblasts, were each sandwiched between two compartments of the vertical diffusion chamber (VDC) (Harvard Apparatus, UK).

    Techniques: Infection, In Vitro, Diffusion-based Assay, MANN-WHITNEY